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At the end of the permeability assay midbrain organoids were removed from the bottom of acceptor compartments, homogenized in DW containing Triton X-100 (10 mg/ml)

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At very low concentrations, peptides are more prone to adsorbing onto the glass walls of the vial, effectively reducing the amount available in solution over time

Also, the stronger absorption can increase the possibility of side effects (though DSIPs side-effect profile remains modestly characterized)

& Arnould, T